Separation of truncated basic fibroblast growth factor from the full-length protein by hydrophobic interaction chromatography

Effective Protein Separation by Coupling Hydrophobic Interaction and Reverse Phase Chromatography for Top-down Proteomics

One of the challenges in proteomics is the proteome's complexity, which necessitates the fractionation of proteins prior to the mass spectrometry (MS) analysis. Despite recent advances in top-down proteomics, separation of intact proteins remains challenging. Hydrophobic interaction chromatography (HIC) appears to be a promising method that provides high-resolution separation of intact proteins...

Modulation of megakaryocytopoiesis by human basic fibroblast growth factor.

Basic fibroblast growth factor (bFGF) may act to modulate hematopoiesis in addition to its effects on mesenchymal cells. We studied the effects of bFGF on human and murine primary marrow megakaryocytes. bFGF modestly enhanced the size of the human megakaryocyte colony-forming unit (CFU-MK) and cell numbers per colony, in combination with interleukin-3 (IL-3) or granulocyte-macrophage colony sti...

Basic fibroblast growth factor promotes the generation of microtubule-associated protein 2-positive cells from microglia.

We recently demonstrated that microglia as multipotential stem cells give rise to microtubule-associated protein 2 (MAP2)-positive and glial fibrillary acidic protein (GFAP)-positive cells and that microglia-derived MAP2-positive cells possess properties of functional neurons. In this study, we investigated the role of fibroblast growth factor (FGF) signaling in the molecular mechanism underlyi...

Effect of Basic Fibroblast Growth Factor on Achilles Tendon Healing in Rabbit

BACKGROUND Tendon injuries are common and it takes a long time for an injured tendon to heal. Adverse phenomena such as adhesion and rupture are associated with these injuries. Finding a method to reduce the time required for healing whichimproves the final outcome, will lead to decreased frequency and intensity of adverse consequences. This study was designed to investigate the effects ...

Hydrophobic Interaction Chromatography